Edition : 2018
Year : 1
Description :
This volume presents the latest developments and techniques used to study the physiopathology, diagnosis, and treatment of pre-eclampsia. Written for clinicians, obstetricians, basic scientists, researchers, and students the chapters in this book provide methods to study placental function using in vitro and ex vivo model systems, comprehensive genetic analysis of pre-eclampsia, identifying critical angiogenic factors associated with the development of pre-eclampsia, and controlled experiments that investigate potential therapies.
Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls, as well as a description of the limitations and advantages of the described techniques.
Cutting-edge and comprehensive, Pre-Eclampsia: Methods and Protocols is a valuable resource for anyone interested in learning more about the critical role of the placenta in the maternal-fetal relationship.
1. Diagnostic Imaging: Ultrasound
2. Biomarker Immunoassays in the Diagnosis of Pre-Eclampsia: Calculating the sFlt1:PlGF Ratio Using the
3. Assessing the Circulating Placental Specific Anti-Angiogenic Protein sFLT-1 e15a in Pre-Eclampsia
4. Role of Activin A in the Pathogenesis of Endothelial Cell Dysfunction in Pre-Eclampsia
5. Genetic Approaches in Pre-Eclampsia
6. Epigenetics and Pre-Eclampsia: Programming of Future Outcomes
7. Inflammatory and Immune System Markers
8. Methods to Enrich Exosomes from Conditioned Media and Biological Fluids
9. Isolation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental Explants
10. Optimized Specific Isolation of Placenta-Derived Exosomes from Maternal Circulation
11. Proteomics Method to Identification of Protein Profiles in Exosomes
12. Harvesting and Characterization of Syncytial Nuclear Aggregates following Culture of First Trimester
13. Use of GATA3 and TWIST1 Immunofluorescence Staining to Assess In Vitro Syncytial Fusion Index
14. Ex Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of their Off-Target Effects
15. Immunohistological Techniques
16. Using a Next Generation Sequencing Approach to Profile MicroRNAs from Human Origin
17. Isolation and Purification of Villous Cytotrophoblast Cells from Term Human Placenta
18. Analyzing Trophoblast Function Using Cell-Based Assays
19. Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and decidua basalis
20. An Electrical Impedance-Based Assay to Examine Functions of Various Placental Cell Types In Vitro
21. In Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental Diseases
22. Measurement of Oxidative Stress-Mitochondrial Function Using the Seahorse System
23. Co-Culture of H295R Adrenocortical Carcinoma and BeWo Choriocarcinoma Cells to Study Feto-Placental Interactions: Focus on Estrogen Biosynthesis
24. Placental Lipid Transport
25. EG-VEGF Maintenance over Early Gestation to Develop a Pregnancy Induced Hypertensive Animal Model
26. Real-Time Blood Pressure Recording Using Radiotelemetry in a Rat Model of Pre-Eclampsia
27. Phase I Pilot Clinical Trial of Antenatal Maternally Administered Melatonin to Decrease the Level of Oxidative Stress in Human Pregnancies Affected by Pre-Eclampsia
28. A Randomized Double-Blinded Placebo-Controlled Intervention Trial of Melatonin for the Prevention of Pre-Eclampsia in Moderate- and High-Risk Women: The MELPOP Trial
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